YqiD

• Description: geranyltransferase
  
  

Gene name	yqiD
Synonyms	ispA
Essential	no
Product	geranyltransferase
Function	biosynthesis of peptidoglycan and teichoic acid
MW, pI	29 kDa, 5.021
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	dxs, yqiC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of lipids

The gene

Basic information

• Locus tag: BSU24280

Expression

Phenotypes of a mutant

The mutation of yqiD predisposes cells to grow without a wall PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In B. subtilis there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane.

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: dimethylallyl pyrophosphate + 2 isopentanyl pyrophosphate -> farnasyl pyrophosphate

• Protein family:

• Paralogous protein(s): HepT

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: None

• Cofactor(s): Magnesium

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:
  • 1RQI: IspA from E. coli. Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate PubMed

• UniProt: P54383

• KEGG entry: [2]

• E.C. number: 2.5.1.10

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M Leaver, P Domínguez-Cuevas, J M Coxhead, R A Daniel, J Errington
Life without a wall or division machine in Bacillus subtilis.
Nature: 2009, 457(7231);849-53
[PubMed:19212404] [WorldCat.org] [DOI] (I p)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

David J Hosfield, Yanming Zhang, Douglas R Dougan, Alexei Broun, Leslie W Tari, Ronald V Swanson, John Finn
Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis.
J Biol Chem: 2004, 279(10);8526-9
[PubMed:14672944] [WorldCat.org] [DOI] (P p)