nfo

168

type IV apurinic/apyrimidinic endonuclease

locus: BSU_25130

Molecular weight: 32.92 kDa

pI: 5.37

Protein length: 297 aa

Gene length: 894 bp

function: repair of oxidative DNA damage in spores

product: type IV apurinic/apyrimidinic endonuclease

essential: no

synonyms: nfo, yqfS

List of homologs in different organisms, belongs to COG0648 (Galperin et al., 2021)

Gene

Coordinates: 2,593,281  2,594,174

Phenotypes of a mutant

• an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]'' [Pubmed|24244006]
• an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is sensitive to radiation [Pubmed|24123749]
• sensitive to blue light-induced DNA damage [pubmed|30054368]
• reduced resistance towards electron beams [pubmed|31948638]

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The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to UniProt)
• binds to 8-oxoG, abasic, and RNA base DNA lesions [pubmed|37250769]

Protein family

• AP endonuclease 2 family (single member, according to UniProt)

Structure

• [PDB|3AAL] (from Geobacillus kaustophilus, 80% identity) [pubmed|21358045]
• [AF|P54476]

Expression and Regulation

Operons

• genes: [gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]

  description: [pubmed|22383849]

Biological materials

Mutant

• GP899 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::''kan'') and GP1502 ([gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
• GP1503 (Δ[gene|38CC84343C47CBF37F007E9056A9248869C396E1|exoA]::''kan'' [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
• MGNA-C462 (yqfS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2460 NBRP B. subtilis, Japan]
• available in [wiki|Mario Pedraza-Reyes]'s lab
• BKE25130 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT,  downstream forward: _UP4_TAAAAAATGGGGGATAACAC
• BKK25130 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT,  downstream forward: _UP4_TAAAAAATGGGGGATAACAC

References

Reviews

• 22933559,38551349

Original Publications

• 24244006,18203828,16237020,12949090,19930460,12486072,21441501,24123749,24914186,21358045,30054368,31948638,37250769