yqfD

168

[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]-dependent sporulation gene, similar to UDP-glucose 4-epimerase

locus: BSU_25350

Molecular weight: 45.57 kDa

pI: 10

Protein length: 398 aa

Gene length: 1197 bp

function: sporulation

product: unknown

essential: no

synonyms: yqfD

List of homologs in different organisms, belongs to COG5872 (Galperin et al., 2021)

Gene

Coordinates: 2,615,452 → 2,616,648

Phenotypes of a mutant

• defect in sporulation [Pubmed|14523133]; some reduction in [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activity and production of small spores [Pubmed|26735940]

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The protein

Structure

• [AF|P54469]

[wiki|Localization]

• polar (Septum) [Pubmed|16479537]

Biological materials

Mutant

• MGNA-C444 (yqfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2442 NBRP B. subtilis, Japan]
• BKE25350 (Δ[gene|E5F5528DA775DE3EE2DD1EC931CDC7E3781E018C|yqfD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCACAACATTTCCCCCTC,  downstream forward: _UP4_CCTATTGTCAGGGAGACTGA
• BKK25350 (Δ[gene|E5F5528DA775DE3EE2DD1EC931CDC7E3781E018C|yqfD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCACAACATTTCCCCCTC,  downstream forward: _UP4_CCTATTGTCAGGGAGACTGA

References

• 14523133,16479537,12662922,15383836,26735940,35638784