txpA

168

toxic peptide, eliminates defective cells from developing biofilms

locus: BSU_26050

Molecular weight: 6.60 kDa

pI: 8.14

Protein length: 59 aa

Gene length: 180 bp

function: toxin

product: toxic peptide

essential: no

synonyms: txpA, yqcR

List of homologs in different organisms

Gene

Coordinates: 2,678,240  2,678,419

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The protein

Catalyzed reaction/ biological activity

• part of a type I toxin/antitoxin system (with [protein|EF79E525504B99CE40B2BD0EE0BB8F8A2E9C7F7B|ratA]) [Pubmed|27450630]
• eliminates defective cells from developing biofilms [Pubmed|27450630]

Structure

• [AF|P54373]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]

  description: [Pubmed|16166525]

  regulation

  • induced by amino acid limitation [Pubmed|27450630]

  regulatory mechanism

  • [protein|EF79E525504B99CE40B2BD0EE0BB8F8A2E9C7F7B|ratA]: antisense RNA, in [regulon|protein:EF79E525504B99CE40B2BD0EE0BB8F8A2E9C7F7B|ratA regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: transcription activation, undefined, in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16166525], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • next to '[protein|search|yonT]', the '[protein|search|txpA]' mRNA has the strongest Shine-Dalgarno region in 'B. subtilis' [PubMed|19210617]

Biological materials

Mutant

• BKE26050 ([gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATTTCACCTCCTTTCA,  downstream forward: _UP4_TAGTTTCTTTTTTAAAAGCT
• BKK26050 ([gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATTTCACCTCCTTTCA,  downstream forward: _UP4_TAGTTTCTTTTTTAAAAGCT

labs

• [wiki|Richard Losick], Harvard University, Cambridge, USA [http://www.mcb.harvard.edu/Losick/ Homepage]

References

Reviews

• 23300472,31075979,38052429

Original Publications

• 16166525,19210617,23300471,27450630