ypgQ

168

Mn²⁺-dependent pyrophosphohydrolase, hydrolyzes (deoxy)ribonucleoside triphosphate [(d)NTP] to (deoxy)ribonucleoside monophosphate and pyrophosphate

locus: BSU_21890

Molecular weight: 22.98 kDa

pI: 5.51

Protein length: 205 aa

Gene length: 618 bp

function: degradation of excessive or abnormal nucleotides

product: Mn²⁺-dependent pyrophosphohydrolase

essential: no

synonyms: ypgQ

List of homologs in different organisms, belongs to COG1418 (Galperin et al., 2021)

Gene

Coordinates: 2,303,920  2,304,537

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The protein

[wiki|Domains]

• [wiki|HD domain] (aa 22-138) [Pubmed|27062940]

[wiki|Cofactors]

• Mn²⁺ [Pubmed|27062940]

Structure

• [PDB|5IHY] [Pubmed|27062940]
• [PDB|5DQV] [Pubmed|27062940]
• [PDB|5DQW] (complexed with ADP) [Pubmed|27062940]
• [AF|P54168]

Biological materials

Mutant

• MGNA-A888 (ypgQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/888 NBRP B. subtilis, Japan]
• BKE21890 ([gene|A0CC440185318F7F8665AF7A8279C79A09E35518|ypgQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCCTTCACAAAAACACCCT,  downstream forward: _UP4_TAATAGAAGGCTTGGAGCGA
• BKK21890 ([gene|A0CC440185318F7F8665AF7A8279C79A09E35518|ypgQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCCTTCACAAAAACACCCT,  downstream forward: _UP4_TAATAGAAGGCTTGGAGCGA

References

• 25005104,27062940