ypeP

168

similar to RNase HI

locus: BSU_21970

Molecular weight: 13.58 kDa

pI: 5.24

Protein length: 226 aa

Gene length: 681 bp

function: unknown

product: unknown

essential: no

synonyms: ypeP

List of homologs in different organisms, belongs to COG0328 (Galperin et al., 2021)

Gene

Coordinates: 2,308,967  2,309,647

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The protein

[wiki|Domains]

• RNase H domain (aa 71-207) (according to UniProt)

Structure

• [AF|P54164]

Biological materials

Mutant

• MGNA-A893 (ypeP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/893 NBRP B. subtilis, Japan]
• BP443 (''ypeP::tet'') available in [wiki|Fabian Commichau]'s lab
• BKE21970 ([gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCTTACCAT,  downstream forward: _UP4_TTAGACCGTAACGGAGATGA
• BKK21970 ([gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCTTACCAT,  downstream forward: _UP4_TTAGACCGTAACGGAGATGA

Expression vectors

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], based on [wiki|pGP380],  expression from plasmid: pBP501 (E. coli amp & B. subtilis E/L) , available in [wiki|Fabian Commichau]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab

References

Research papers

• 29084857