czrA

168

transcriptional repressor ([wiki|ArsR family]), [gene|5BC6B02770E74FF6D453742832574A87BB2369B8|cadA] and [gene|7FB5BCC16D688465A820F436F657C49625257884|czcD]

locus: BSU_19120

Molecular weight: 12.24 kDa

pI: 5.72

Protein length: 107 aa

Gene length: 324 bp

function: regulation of resistance against toxic metal cations

product: transcriptional repressor ([wiki|ArsR family])

essential: no

synonyms: czrA, yozA

List of homologs in different organisms, belongs to COG0640 (Galperin et al., 2021)

Gene

Coordinates: 2,084,786  2,085,109

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The protein

Protein family

• [wiki|ArsR family]

[wiki|Domains]

• [wiki|HTH arsR-type domain] (aa 14-107) (according to UniProt)

Structure

• [PDB|1R1U] (the apo-repressor from ''Staph. aureus'', 49% identity, 82% similarity) [Pubmed|14568530]
• [PDB|1R1V] (the Zn(II) form from ''Staph. aureus'', 49% identity, 82% similarity) [Pubmed|14568530]
• [PDB|2KJB] (the DNA-bound form from ''Staph. aureus'', 49% identity, 82% similarity) [Pubmed|19822742]
• [AF|O31844]

Effectors of protein activity

• DNA-binding activity of CzrA is inactivated by high concentrations of toxic metal ions (induction)

Expression and Regulation

Operons

• genes: [gene|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|czrA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B412 (yozA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1411 NBRP B. subtilis, Japan]
• BKE19120 ([gene|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|czrA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTATCACCTTTCTTT,  downstream forward: _UP4_TGAAAAAGACATCCTTGCTT
• BKK19120 ([gene|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|czrA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTATCACCTTTCTTT,  downstream forward: _UP4_TGAAAAAGACATCCTTGCTT

References

• 15948947,16430705,14568530,19822742,25213752,22007899