Difference between revisions of "YodF"

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* '''Description:''' similar to proline permease <br/><br/>
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* '''Description:''' similar to amino acid permease <br/><br/>
  
 
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{| align="right" border="1" cellpadding="2"  
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 +
** in some database, the protein is annotated as being similar to proline permease, however, it is not involved in proline transport  {{PubMed|24142252}}
  
* '''Protein family:'''
+
* '''Protein family:''' solute:sodium symporter family (SSS family)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Mutant:'''
 
* '''Mutant:'''
 +
** available in [[Erhard Bremer]]'s lab {{PubMed|24142252}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
  
<pubmed>12823818,,12823818,12107147 18763711, </pubmed>
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<pubmed>12823818,,12823818,12107147 18763711, 24142252</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:26, 28 November 2013

  • Description: similar to amino acid permease

Gene name yodF
Synonyms
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yodF
MW, pI 53 kDa, 9.682
Gene length, protein length 1488 bp, 496 aa
Immediate neighbours yoyD, ctpA
Sequences Protein DNA DNA_with_flanks
Genetic context
YodF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YodF expression.png















Categories containing this gene/protein

transporters/ other, putative amino acid transporter, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU19580

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • in some database, the protein is annotated as being similar to proline permease, however, it is not involved in proline transport PubMed
  • Protein family: solute:sodium symporter family (SSS family)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by casamino acids PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Adrienne Zaprasis, Tamara Hoffmann, Lorena Stannek, Katrin Gunka, Fabian M Commichau, Erhard Bremer
The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis.
J Bacteriol: 2014, 196(3);515-26
[PubMed:24142252] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)