desR

desR
168

two-component response regulator, regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]

locus
BSU_19200
Molecular weight
22.03 kDa
pI
4.89
Protein length
Gene length
function
regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
product
two-component response regulator
essential
no
synonyms
desR, yocG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2197 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,091,705 → 2,092,304
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The protein
Catalyzed reaction/ biological activity
transcription activation of the ''[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]'' operon when phosphorylated by [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]
[wiki|Domains]
[wiki|Response regulatory domain] (aa 3-117) (according to UniProt)
[wiki|HTH luxR-type domain] (aa 131-196) (according to UniProt)
Structure
[PDB|5IUJ] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphotransfer state with low Mg2+ [20 mM]) [Pubmed|27938660]
[PDB|5IUK] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphotransfer state with high Mg2+ [150 mM] and BeF3) [Pubmed|27938660]
[PDB|5IUN] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphatase state) [Pubmed|27938660]
[AF|O34723]
Modification
phosphorylated by [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK] on an Asp residue
Paralogous protein(s)
[protein|5BB2B90D5DDD50DD9B4EF9E69925FCBFA642222B|yvfU]
Expression and Regulation
Operons
genes
[gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]
description
[Pubmed|11285232]
regulation
induced by cold shock (12-fold) [Pubmed|12399512]
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[gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]→[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]

2024-02-06 18:54:56

ghost

60

25b9128a8b7f56b50905080b061f40a8b0014210

F2EF1E99AB7B6CD97A042B3BC67273F913364739

Biological materials
Mutant
MGNA-A320 (yocG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/320 NBRP B. subtilis, Japan]
BKE19200 (Δ[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATCATATAAGCCATCCTTT,  downstream forward: _UP4_TAAAAAAGGATCTTGGCATC
BKK19200 (Δ[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATCATATAAGCCATCCTTT,  downstream forward: _UP4_TAAAAAAGGATCTTGGCATC
labs
[wiki|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
References
10094672,12399512,19595746,11285232,17087771,12207704,25406381,27938660,29269314,36693130

F08BE4ACC0ECE1416B012CA72B027423D4D9B00A

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Time of last update: 2024-03-28 20:00:21

Author of last update: Jstuelk