yoaJ

168

bacterial expansin, binds cellulose, required for the colonization of maize roots, putative cell wall hydrolase (N-acetylglucosamidase)

locus: BSU_18630

Molecular weight: 25.49 kDa

pI: 9.45

Protein length: 232 aa

Gene length: 699 bp

function: interaction with plant roots

product: expansin

essential: no

synonyms: yoaJ, EXLX1

List of homologs in different organisms, belongs to COG4305 (Galperin et al., 2021)

Gene

Coordinates: 2,032,927  2,033,625

Phenotypes of a mutant

• strongly reduced ability to colonize maize  roots [Pubmed|18971341]

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The protein

Protein family

• expansin [Pubmed|18971341]

[wiki|Domains]

• Expansin-like EG45 domain (aa 58-127) (according to UniProt)

Structure

• [PDB|3D30] [Pubmed|18971341]
• [AF|O34918]

[wiki|Localization]

• extracellular [Pubmed|18971341]

Biological materials

Mutant

• MGNA-A839 (yoaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/839 NBRP B. subtilis, Japan]
• BKE18630 ([gene|17094CBA2DDFB82B5FC064AADB85DAB60A15B2C0|yoaJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTGGTCCTCCTCAAA,  downstream forward: _UP4_TAAAAAATACGAAACAGCGG
• BKK18630 ([gene|17094CBA2DDFB82B5FC064AADB85DAB60A15B2C0|yoaJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTGGTCCTCCTCAAA,  downstream forward: _UP4_TAAAAAATACGAAACAGCGG

References

Reviews

• 25833181,27365165

Original Publications

• 19058186,15604683,16984999,22927418,18971341,21454649,12354229,23053073,24755657,22949138,26521249,26751268,27365165,30221235,31271651,31689330