Difference between revisions of "YoaA"

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Line 112: Line 112:
  
 
* '''Mutant:'''
 
* '''Mutant:'''
 +
** GP1248 (''yoaA''::''tet''), available in [[Stülke]] lab
 +
** GP1255 (''acuA''::''kan'' ''yjck''::''cat'' ''ynaD''::''spec'' ''yoaA''::''tet''), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 08:25, 26 July 2011

  • Description: similar to ribosomal-protein-alanine N-acetyltransferase

Gene name yoaA
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 18 kDa, 5.92
Gene length, protein length 495 bp, 165 aa
Immediate neighbours yoxB, yoaB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YoaA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

translation, protein modification, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU18530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP1248 (yoaA::tet), available in Stülke lab
    • GP1255 (acuA::kan yjck::cat ynaD::spec yoaA::tet), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)