YmcB

• Description: tRNA methylthiotransferase
  
  

Gene name	ymcB
Synonyms
Essential	no
Product	tRNA methylthiotransferase
Function	tRNA maturation
MW, pI	57 kDa, 6.873
Gene length, protein length	1527 bp, 509 aa
Immediate neighbours	kbl, ymcA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU17010

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

translation

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA PubMed

• Protein family: MiaB subfamily (according to Swiss-Prot)

• Paralogous protein(s): YqeV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31778

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1109 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Brian P Anton, Susan P Russell, Jason Vertrees, Simon Kasif, Elisabeth A Raleigh, Patrick A Limbach, Richard J Roberts
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.
Nucleic Acids Res: 2010, 38(18);6195-205
[PubMed:20472640] [WorldCat.org] [DOI] (I p)