fakA

fakA
168

fatty acid kinase

locus
BSU_15840
Molecular weight
59.32 kDa
pI
4.28
Protein length
Gene length
function
phosphorylation of fatty acids
product
fatty acid kinase
essential
no
synonyms
fakA, yloV

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1461 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,656,442  1,658,103
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The protein
Catalyzed reaction/ biological activity
phosphorylation of fatty acids (bound to [protein|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|degV] or [protein|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]) [pubmed|30429218]
[wiki|Domains]
DhaL domain (aa 9-201) (according to UniProt)
Structure
[PDB|7RM7] (from S. aureus, N-terminal domain) (67% identity)
[PDB|6W6B] (from S. aureus, C-terminal domain) (48% identity)
[AF|O34751]
Modification
phosphorylated on Arg-255 [Pubmed|22517742]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
Expression and Regulation
Operons
genes
[gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]
description
[Pubmed|22383849]
Open in new tab

[gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]→[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]

2024-03-18 23:05:15

Bzhu

62

59df383c358e285f8f394d9d07619671e9b5d639

60743BCE1ECE1AB944532A871551DEAAB9A8E78C

Biological materials
Mutant
MGNA-B137 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1136 NBRP B. subtilis, Japan]
GP1466 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::''cat''), available in [wiki|Jörg Stülke]'s lab
GP1467 ([gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB] - [gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
BKE15840 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATAGACAATCCTACTCCTC,  downstream forward: _UP4_TAGAAGGGCAATTTGCCCTT
BKK15840 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATAGACAATCCTACTCCTC,  downstream forward: _UP4_TAGAAGGGCAATTTGCCCTT
Expression vectors
pGP3623 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
pGP3624 expression of Strep-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA] by [wiki|pGP380] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3631 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP3643 (C-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP3780 [gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]-3xFLAG spc (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
22383849,17981983,22517742,28579978,30429218,29581406,32671874

5DE73E8005AFFD30EB84696B2C2474899C6D1982

Page visits: 4246

Time of last update: 2024-03-28 01:24:44

Author of last update: Dennis.wicke