YloU

• Description: similar to alkaline-shock protein
  
  

Gene name	yloU
Synonyms
Essential	yes PubMed
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yloU
MW, pI	13 kDa, 7.077
Gene length, protein length	360 bp, 120 aa
Immediate neighbours	rpmB, yloV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function, essential genes

The gene

Basic information

• Locus tag: BSU15830

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: asp23 family (according to Swiss-Prot)

• Paralogous protein(s): YqhY

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34318

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yloU-yloV PubMed

• Expression browser: yloU PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP1465 (yloU::cat), available in Jörg Stülke's lab
  • GP1467 (yloU - yloV::cat), available in Jörg Stülke's lab

• Expression vector:
  • GP1476 (chromosomal yloU-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
  • pGP1323 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
  • pGP1324 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:
  • pBP43-yloU-Gfp, available in Jörg Stülke's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody: