sirB

168

sirohydrochlorin ferrochelatase

locus: BSU_15620

Molecular weight: 28.69 kDa

pI: 6.06

Protein length: 261 aa

Gene length: 786 bp

function: siroheme biosynthesis, sulfite reduction

product: sirohydrochlorin ferrochelatase

essential: no

synonyms: sirB, ylnE

List of homologs in different organisms, belongs to COG2138 (Galperin et al., 2021)

Gene

Coordinates: 1,634,837  1,635,622

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The protein

Catalyzed reaction/ biological activity

• insertion of Fe2+ into the substrate sirohydrochlorin (SHC) in siroheme biosynthesis [pubmed|30778451]
• 2 H⁺ + siroheme --> Fe²⁺ + sirohydrochlorin (according to UniProt)

Protein family

• CbiX family (single member, according to UniProt)

Structure

• Apo-form: [PDB|5ZT8] [pubmed|30778451]
• Co²⁺ bound: [PDB|5ZT7] ([pubmed|30778451]), [PDB|6JV6]
• Ni²⁺ bound: [PDB|5ZT9]
• Fe³⁺ bound: [PDB|5ZTA]
• [AF|O34632]

Biological materials

Mutant

• MGNA-B369 (ylnE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1368 NBRP B. subtilis, Japan]
• BKE15620 ([gene|9349C4646A56F933041EBE750F1BE86BAE7A60D9|sirB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATATAAAATTGCTTGTTTCA,  downstream forward: _UP4_CAATTTGATTTTGATGGAGG
• BKK15620 ([gene|9349C4646A56F933041EBE750F1BE86BAE7A60D9|sirB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATATAAAATTGCTTGTTTCA,  downstream forward: _UP4_CAATTTGATTTTGATGGAGG

References

• 16267287,11004190,12107147,18039762,30778451