YlmB

• Description: N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase

Gene name	ylmB
Synonyms
Essential	no
Product	N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase
Function	thiamine salvage
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	47 kDa, 4.981
Gene length, protein length	1278 bp, 426 aa
Immediate neighbours	ylmA, ylmC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU15350

Expression

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine + H2O = formate + 4-amino-5-aminomethyl-2-methylpyrimidine (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): YodQ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):Binds 2 zinc or cobalt ions per subunit (according to Swiss-Prot)

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31724

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Amy Haas Jenkins, Ghislain Schyns, Sébastien Potot, Guangxing Sun, Tadhg P Begley
A new thiamin salvage pathway.
Nat Chem Biol: 2007, 3(8);492-7
[PubMed:17618314] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536] [WorldCat.org] [DOI] (P p)