Difference between revisions of "YlaN"

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(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 +
** IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab
 +
** IPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
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* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  
  
* '''Antibody:'''
+
* '''Antibody:''' available in [[Jörg Stülke]]'s lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 16:33, 13 August 2013

  • Description: unknown

Gene name ylaN
Synonyms
Essential yes PubMed
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ylaN
MW, pI 10 kDa, 4.615
Gene length, protein length 279 bp, 93 aa
Immediate neighbours ylaM, ftsW
Sequences Protein DNA DNA_with_flanks
Genetic context
YlaN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YlaN expression.png















Categories containing this gene/protein

essential genes, proteins of unknown function

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14840

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0358 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • IPTG inducible expression of Strep-ylaN in E. coli: pGP2579 (in pGP172), available in Jörg Stülke's lab
    • IPTG inducible expression of His-ylaN in E. coli: pGP2583 (in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Your additional remarks

References

Fabian M Commichau, Nico Pietack, Jörg Stülke
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
Mol Biosyst: 2013, 9(6);1068-75
[PubMed:23420519] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Ling Xu, Svetlana E Sedelnikova, Patrick J Baker, Alison Hunt, Jeff Errington, David W Rice
Crystal structure of S. aureus YlaN, an essential leucine rich protein involved in the control of cell shape.
Proteins: 2007, 68(2);438-45
[PubMed:17469204] [WorldCat.org] [DOI] (I p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)