ohrB

168

general stress protein

locus: BSU_13160

Molecular weight: 14.00 kDa

pI: 4

Protein length: 136 aa

Gene length: 411 bp

function: organic peroxide resistance

product: unknown

essential: no

synonyms: ohrB, yzzE, ykzA

List of homologs in different organisms, belongs to COG1764 (Galperin et al., 2021)

Gene

Coordinates: 1,382,020 → 1,382,430

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The protein

Protein family

• osmC/ohr family (with [protein|869E46AECF6249610F8541E403D834284B62171D|ohrA] and [protein|0785B4C70C3DD00EFB8BCDD91EF4F98EF4372186|ymaD], according to UniProt)

Structure

• [PDB|2BJO] [pubmed|18084074]
• [AF|P80242]

Modification

• Cys55-Cys119 form catalytic disulfide bond [Pubmed|18084074]

Paralogous protein(s)

• [protein|869E46AECF6249610F8541E403D834284B62171D|ohrA]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|C36DD4B670D803C5700428F62E6CA7ED127E4B41|ohrB]-[gene|9C8CF0AC3581D5FAA43A44D8B893D46E1A67C219|ykzN]

  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

• genes: [gene|C36DD4B670D803C5700428F62E6CA7ED127E4B41|ohrB]

  description: [Pubmed|9696771]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|15805528]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• GP1728 [gene|C36DD4B670D803C5700428F62E6CA7ED127E4B41|ohrB]::spec trpC2 available at Jörg Stülkes lab
• MGNA-A751 (ykzA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/751 NBRP B. subtilis, Japan]
• BKE13160 (Δ[gene|C36DD4B670D803C5700428F62E6CA7ED127E4B41|ohrB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCCAACCTCCTTA,  downstream forward: _UP4_TAATGACCGGCATCCTGAGG
• BKK13160 (Δ[gene|C36DD4B670D803C5700428F62E6CA7ED127E4B41|ohrB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCCAACCTCCTTA,  downstream forward: _UP4_TAATGACCGGCATCCTGAGG

References

• 18084074,9696771,11418552,15805528