queE

168

7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine

locus: BSU_13740

Molecular weight: 26.97 kDa

pI: 4.96

Protein length: 243 aa

Gene length: 732 bp

function: tRNA modification

product: 7-carboxy-7-deazaguanine (CDG) synthase

essential: no

synonyms: queE, ykvL

List of homologs in different organisms, belongs to COG0602 (Galperin et al., 2021)

Gene

Coordinates: 1,440,542 → 1,441,273

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The protein

Catalyzed reaction/ biological activity

• radical-mediated ring rearrangement required to convert 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) into 7-carboxy-7-deazaguanine (CDG) [pubmed|29303575]

Protein family

• [wiki|Radical SAM superfamily] (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]
• S-adenosyl-L-methionine, Mg(2+) [Pubmed|23194065]

Structure

• [PDB|5TGS] [pubmed|28045519]
• [AF|O31677]

Expression and Regulation

Operons

• genes: [gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]

  description: [Pubmed|14660578]

  regulation

  • repressed in the presence of queuosine ([wiki|preQ1 riboswitch]) [Pubmed|19285444]

  regulatory mechanism

  • preQ1 riboswitch: RNA switch, in the absence of queuosine [Pubmed|19285444], in [regulon|other_regulator:preQ1 riboswitch|preQ1 riboswitch]

Biological materials

Mutant

• MGNA-A790 (ykvL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/790 NBRP B. subtilis, Japan]
• BKE13740 (Δ[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAAAATTTCTAATACAGGAA,  downstream forward: _UP4_GTATAATAGAAAGGAAGATG
• BKK13740 (Δ[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAAAATTTCTAATACAGGAA,  downstream forward: _UP4_GTATAATAGAAAGGAAGATG

References

• 14660578,19285444,19354300,17384645,24362703,23194065,25933252,29303575,28045519