yktC

168

inositol monophosphatase and 5'-nucleotidase with preference for GMP and IMP

locus: BSU_14670

Molecular weight: 29.61 kDa

pI: 5.09

Protein length: 265 aa

Gene length: 798 bp

function: unknown

product: nucleotidase

essential: no

ec: 3.1.3.25

synonyms: yktC, suhB

List of homologs in different organisms, belongs to COG0483 (Galperin et al., 2021)

Gene

Coordinates: 1,537,441  1,538,238

Phenotypes of a mutant

• sensitive to diamide stress [Pubmed|27784292]

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The protein

Catalyzed reaction/ biological activity

• myo-inositol phosphate + H₂O --> myo-inositol + phosphate (according to UniProt)

Protein family

• inositol monophosphatase superfamily (single member, according to UniProt)

Structure

• [PDB|5I3S] (from Staphylococcus aureus, 51% identity)
• [AF|Q45499]

Expression and Regulation

Operons

• genes: [gene|AD151804FC145854DB6BAA12CD52758FB1C3882F|yktC]-[gene|A313B197D6D885EEE7F739E1F137794A8E3AD8F9|ykzC]

  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-A908 (yktC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/908 NBRP B. subtilis, Japan]
• BKE14670 ([gene|AD151804FC145854DB6BAA12CD52758FB1C3882F|yktC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCGTTCGTACCTCTCTT,  downstream forward: _UP4_TAGGCCATTTGAGCAGGATG
• BKK14670 ([gene|AD151804FC145854DB6BAA12CD52758FB1C3882F|yktC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCGTTCGTACCTCTCTT,  downstream forward: _UP4_TAGGCCATTTGAGCAGGATG

References

• 19935659,26577401,27784292