mtnX

168

2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase

locus: BSU_13600

Molecular weight: 26.85 kDa

pI: 4.66

Protein length: 235 aa

Gene length: 708 bp

function: methionine salvage

product: 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase

essential: no

synonyms: mtnX, ykrX

List of homologs in different organisms, belongs to COG4359 (Galperin et al., 2021)

Gene

Coordinates: 1,428,275  1,428,982

Advertisement

The protein

Catalyzed reaction/ biological activity

• 2-hydroxy-5-methylsulfanyl-3-oxopent-1-enyl phosphate + H₂O --> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate (according to UniProt)

Protein family

• [wiki|HAD superfamily] (according to UniProt)

Structure

• [PDB|2FEA] [pubmed|17654724]
• [AF|O31667]

Biological materials

Mutant

• MGNA-A785 (ykrX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/785 NBRP B. subtilis, Japan]
• BKE13600 ([gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG,  downstream forward: _UP4_GAGAATGTAAAGGAGGTACA
• BKK13600 ([gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG,  downstream forward: _UP4_GAGAATGTAAAGGAGGTACA

References

• 12022921,11914366,12107147,15102328,12787499,18039762,17654724