thiV

168

thiamine [wiki|ECF transporter] (membrane protein, thiamine-specific S protein)

locus: BSU_13230

Molecular weight: 21.02 kDa

pI: 9.89

Protein length: 199 aa

Gene length: 600 bp

function: thiamine uptake

product: thiamine [wiki|ECF transporter] (membrane protein, thiamine-specific S protein)

essential: no

synonyms: thiV, ykoE

List of homologs in different organisms, belongs to COG4721 (Galperin et al., 2021)

Gene

Coordinates: 1,390,439  1,391,038

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The protein

Structure

• [PDB|5EDL] [pubmed|27447050]
• [AF|O34738]

[wiki|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]

  description: [Pubmed|16291685]

  regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• MGNA-A756 (ykoE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/756 NBRP B. subtilis, Japan]
• BKE13230 ([gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTACTTTCCAGCTTT,  downstream forward: _UP4_AAGAAACGGAAGCGGGCGTC
• BKK13230 ([gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTACTTTCCAGCTTT,  downstream forward: _UP4_AAGAAACGGAAGCGGGCGTC

References

• 10092453,16291685,12376536,18763711,27447050