Difference between revisions of "YkgB"

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* '''Antibody:'''
 
* '''Antibody:'''
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* '''FLAG-tag construct:''' GP1402 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 13:57, 22 February 2011

  • Description: putative 6-phosphogluconolactonase, general stress protein

Gene name ykgB
Synonyms
Essential no
Product putative 6-phosphogluconolactonase
Function pentose phosphate pathway
MW, pI 38 kDa, 5.154
Gene length, protein length 1047 bp, 349 aa
Immediate neighbours ykfD, ykgA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkgB context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

general stress proteins (controlled by SigB), carbon core metabolism

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU13010

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: the corresponding protein of E. coli is the 6-phosphogluconolactonase PubMed
  • Protein family: cycloisomerase 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:
  • FLAG-tag construct: GP1402 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

PubMed

Lynn C Thomason, Donald L Court, Atin R Datta, Rita Khanna, Judah L Rosner
Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase.
J Bacteriol: 2004, 186(24);8248-53
[PubMed:15576773] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)