yjmC

168

similar to ureidoglycolate dehydrogenase, may be involved in galacturonate utilization

locus: BSU_12320

Molecular weight: 36.32 kDa

pI: 5.67

Protein length: 337 aa

Gene length: 1014 bp

function: unknown

product: unknown

essential: no

synonyms: yjmC

List of homologs in different organisms, belongs to COG2055 (Galperin et al., 2021)

Gene

Coordinates: 1,303,423  1,304,436

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The protein

Protein family

• LDH2/MDH2 oxidoreductase family (single member, according to UniProt)

Structure

• [PDB|4H8A] (ureidoglycolate dehydrogenase from E. coli, 41% identity) [pubmed|23284870]
• [AF|O34736]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• The gene is annotated in KEGG as an ortholog of malate dehydrogenase EC 1.1.1.37. but it is marked as uncharacterized oxidoreductase (EC 1.1.1.-) in Swiss-Prot. In MetaCyc it is marked as similar to malate dehydrogenase. As the paper by Mekjian et al. [Pubmed|9882655] suggests this gene is more likely to be involved in the glucuronate pathway (for which EC 1.1.1.37 is not a member), no literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

Biological materials

Mutant

• MGNA-A367 (yjmC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/367 NBRP B. subtilis, Japan]
• BKE12320 ([gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCAAGTGCCTCCTTCCT,  downstream forward: _UP4_TTCTTAAAAAGCAGGTGAGT
• BKK12320 ([gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCAAGTGCCTCCTTCCT,  downstream forward: _UP4_TTCTTAAAAAGCAGGTGAGT

References

• 9579062,9882655,10666464,19935659,27941785,23284870