thiD

168

4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase

locus: BSU_11710

Molecular weight: 28.97 kDa

pI: 5.71

Protein length: 271 aa

Gene length: 816 bp

function: biosynthesis of thiamine pyrophosphate (TPP)

product: 4-amino-5-hydroxymethyl-2-methylpyrimidinepyrophosphate kinase

essential: no

ec: 2.7.1.49

synonyms: thiD, yjbV

List of homologs in different organisms, belongs to COG0351 (Galperin et al., 2021)

Gene

Coordinates: 1,246,837  1,247,652

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The protein

Catalyzed reaction/ biological activity

• 4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H⁺ (according to UniProt)
• 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP --> 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP (according to UniProt)

Protein family

• ThiD family (with [protein|FEDD589EA9982856730E809F21B7930F688FDC42|pdxK], according to UniProt)

Structure

• [PDB|2I5B] [Pubmed|16978644]
• [AF|O31620]

Paralogous protein(s)

• [protein|FEDD589EA9982856730E809F21B7930F688FDC42|pdxK]

Biological materials

Mutant

• MGNA-B167 (yjbV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1166 NBRP B. subtilis, Japan]
• BKE11710 ([gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTCATTACACTACACTCCT,  downstream forward: _UP4_TGAGATCACCAGCTGATGGT
• BKK11710 ([gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTCATTACACTACACTCCT,  downstream forward: _UP4_TGAGATCACCAGCTGATGGT

References

Reviews

• 19348578,10382260

Original Publications

• 14973012,16978644