yisP

168

farnesyl diphosphate phosphatase, production of farnesol

locus: BSU_10810

Molecular weight: 31.35 kDa

pI: 7.26

Protein length: 274 aa

Gene length: 825 bp

function: control of [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC] activity

product: farnesyl diphosphate phosphatase

essential: no

synonyms: yisP, yucD

List of homologs in different organisms, belongs to COG1562 (Galperin et al., 2021)

Gene

Coordinates: 1,159,922 → 1,160,746

Phenotypes of a mutant

• complete loss of pellicle-forming ability [Pubmed|20713508]
• reduced [wiki|protein secretion] [Pubmed|23651456]

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The protein

Catalyzed reaction/ biological activity

• farnesyl diphosphate -→ farnesol [Pubmed|25308276]

Protein family

• phytoene/squalene synthase family (single member, according to UniProt)

[wiki|Cofactors]

• NADH  [Pubmed|20713508]

Structure

• [PDB|3WE9] [Pubmed|25308276]
• [AF|O06728]

Biological materials

Mutant

• MGNA-B190 (yisP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1189 NBRP B. subtilis, Japan]
• BKE10810 (Δ[gene|E5F32D960F6FF50A7B0E4B268D2296FE1A291512|yisP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGCTTCTTCAAGTCTAG,  downstream forward: _UP4_TAAAAAACACCCGGCCTTGA
• BKK10810 (Δ[gene|E5F32D960F6FF50A7B0E4B268D2296FE1A291512|yisP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGCTTCTTCAAGTCTAG,  downstream forward: _UP4_TAAAAAACACCCGGCCTTGA

References

Reviews

• 25457121,25652542

Original Publications

• 20713508,19935659,23651456,23295493,25308276,26577401