yisI

168

[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P phosphatase, control of the [wiki|phosphorelay]

locus: BSU_10730

Molecular weight: 6.54 kDa

pI: 5.12

Protein length: 56 aa

Gene length: 171 bp

function: control of [wiki|sporulation] initiation

product: [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P phosphatase

essential: no

synonyms: yisI

List of homologs in different organisms, belongs to COG5804 (Galperin et al., 2021)

Gene

Coordinates: 1,150,850  1,151,020

Advertisement

The protein

Protein family

• spo0E family (with [protein|search|ynzd ]and [protein|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E], according to UniProt)

Structure

• [PDB|2C0S] (from B. anthracis, corresponds to aa 1 ... 41 of YisI, 44% identity) [pubmed|17001075]
• [AF|O06722]

Biological materials

Mutant

• BKE10730 ([gene|022933D89666CDD2ACE39BCBB78D349BAF8E899B|yisI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACGCTATCTCCAATTC,  downstream forward: _UP4_TAAATCATTTTCTATAACAA
• BKK10730 ([gene|022933D89666CDD2ACE39BCBB78D349BAF8E899B|yisI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACGCTATCTCCAATTC,  downstream forward: _UP4_TAAATCATTTTCTATAACAA

References

• 11679073,20525796,27542896,17001075