YhfU

• Description: S protein of biotin ECF transporter
  
  

Gene name	yhfU
Synonyms	bioY
Essential	no
Product	S protein of biotin ECF transporter
Function	uptake of biotin
Gene expression levels in SubtiExpress: yhfU
Interactions involving this protein in SubtInteract: YhfU
Metabolic function and regulation of this protein in SubtiPathways: YhfU
MW, pI	19 kDa, 10.681
Gene length, protein length	558 bp, 186 aa
Immediate neighbours	yhfT, hemAT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ECF transporter, biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

• Locus tag: BSU10370

Database entries

• BsubCyc: BSU10370

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: uptake of biotin PubMed

• Protein family: bioY family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • YbaF-YhfU PubMed

• Localization:
  • membrane associated PubMed

Database entries

• BsubCyc: BSU10370

• Structure: 4DVE (BioY from Lactococcus lactis, 35% identity, 71% similarity) PubMed

• UniProt: O07620

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yhfU PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of biotin (BirA) PubMed

• Regulatory mechanism:
  • BirA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Dirk J Slotboom
Structural and mechanistic insights into prokaryotic energy-coupling factor transporters.
Nat Rev Microbiol: 2014, 12(2);79-87
[PubMed:24362466] [WorldCat.org] [DOI] (I p)

Guus B Erkens, Maria Majsnerowska, Josy ter Beek, Dirk Jan Slotboom
Energy coupling factor-type ABC transporters for vitamin uptake in prokaryotes.
Biochemistry: 2012, 51(22);4390-6
[PubMed:22574898] [WorldCat.org] [DOI] (I p)

Steven Lin, John E Cronan
Closing in on complete pathways of biotin biosynthesis.
Mol Biosyst: 2011, 7(6);1811-21
[PubMed:21437340] [WorldCat.org] [DOI] (I p)

Thomas Eitinger, Dmitry A Rodionov, Mathias Grote, Erwin Schneider
Canonical and ECF-type ATP-binding cassette importers in prokaryotes: diversity in modular organization and cellular functions.
FEMS Microbiol Rev: 2011, 35(1);3-67
[PubMed:20497229] [WorldCat.org] [DOI] (I p)