Difference between revisions of "YfmL"

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|style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase
 
|style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU07430 yfmL]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07430 yfmL]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.602   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.602   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfmM]]'', ''[[yfmK]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfmM]]'', ''[[yfmK]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12572&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU07430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU07430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU07430 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yfmL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yfmL_context.gif]]

Revision as of 12:20, 13 May 2013

Gene name yfmL
Synonyms
Essential no
Product DEAD-box RNA helicase
Function RNA helicase
Gene expression levels in SubtiExpress: yfmL
MW, pI 42 kDa, 9.602
Gene length, protein length 1128 bp, 376 aa
Immediate neighbours yfmM, yfmK
Sequences Protein DNA Advanced_DNA
Genetic context
YfmL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YfmL expression.png















Categories containing this gene/protein

DEAD-box RNA helicases, translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU07430

Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20°C) PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: yfmL (according to DBTBS)
  • Regulation:
    • expression declines in the stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1053 (erm), available in the Stülke lab
  • Expression vector:
    • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
    • B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651] [WorldCat.org] [DOI] (I p)