YfmL

• Description: DEAD-box RNA helicase
  
  

Gene name	yfmL
Synonyms
Essential	no
Product	DEAD-box RNA helicase
Function	RNA helicase
Gene expression levels in SubtiExpress: yfmL
MW, pI	42 kDa, 9.602
Gene length, protein length	1128 bp, 376 aa
Immediate neighbours	yfmM, yfmK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DEAD-box RNA helicases, translation

The gene

Basic information

• Locus tag: BSU07430

Phenotypes of a mutant

• poor growth at low temperatures (16 to 20°C) PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: RNA helicase

• Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
• Paralogous protein(s): CshA, CshB, DeaD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34750

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfmL (according to DBTBS)

• Expression browser: yfmL PubMed

• Sigma factor:

• Regulation:
  • expression declines in the stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1053 (erm), available in the Stülke lab

• Expression vector:
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab
• lacZ fusion:

• GFP fusion:
  • B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct:
  • GP1066 (spc, based on pGP1331), available in Jörg Stülke's

• Antibody:

References

Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651] [WorldCat.org] [DOI] (I p)