Difference between revisions of "YfmL"

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(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
       
+
** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], expression from the native chromomsomal site: GP1069 (spc), available in [[Jörg Stülke]]'s lab       
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
* '''GFP fusion:''' B. subtilis GP1082 yfmL-yfp spc, available in [[Stülke]] lab,
+
* '''GFP fusion:'''  
 +
** B. subtilis GP1082 yfmL-yfp spc, available in [[Jörg Stülke]]'s lab
  
* '''two-hybrid system:'''  B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+
* '''two-hybrid system:'''  B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
  
* '''FLAG-tag construct:''' GP1066 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
+
* '''FLAG-tag construct:'''  
 +
** GP1066 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 12:39, 20 September 2012

Gene name yfmL
Synonyms
Essential no
Product DEAD-box RNA helicase
Function RNA helicase
Gene expression levels in SubtiExpress: yfmL
MW, pI 42 kDa, 9.602
Gene length, protein length 1128 bp, 376 aa
Immediate neighbours yfmM, yfmK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YfmL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YfmL expression.png
























Categories containing this gene/protein

DEAD-box RNA helicases

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU07430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: yfmL (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1053 (erm), available in the Stülke lab
  • Expression vector:
    • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
    • B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References