Difference between revisions of "YfmL"

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(Biological materials)
Line 114: Line 114:
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
 +
** B. subtilis GP1081 yfmL-yfp spc, available in [[Stülke]] lab,
  
 
* '''two-hybrid system:'''  B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
 
* '''two-hybrid system:'''  B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab

Revision as of 11:49, 25 March 2011

Gene name yfmL
Synonyms
Essential no
Product DEAD-box RNA helicase
Function RNA helicase
MW, pI 42 kDa, 9.602
Gene length, protein length 1128 bp, 376 aa
Immediate neighbours yfmM, yfmK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YfmL context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DEAD-box RNA helicases

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU07430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: yfmL (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
    • B. subtilis GP1081 yfmL-yfp spc, available in Stülke lab,
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References