yflS

168

malate transporter

locus: BSU_07570

Molecular weight: 51.27 kDa

pI: 9.74

Protein length: 478 aa

Gene length: 1437 bp

function: malate uptake

product: malate transporter

essential: no

synonyms: yflS

List of homologs in different organisms, belongs to COG0471 (Galperin et al., 2021)

Gene

Coordinates: 829,382  830,818

RNA

additional information

• the mRNA is stabilized by basepairing with the RoxS RNA [pubmed|28436820]
• non-protected mRNA is subject to degradation by by [wiki|rnjA|RNase J1] [pubmed|28436820]

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The protein

Catalyzed reaction/ biological activity

• uptake of malate

Protein family

• SLC13A/DASS transporter (TC 2.A.47) family (single member, according to UniProt)

Structure

• [AF|O34726]

[wiki|Localization]

• cell membrane (according to UniProt)
• spore membrane [pubmed|30602489]

Biological materials

Mutant

• MGNA-C249 (yflS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2247 NBRP B. subtilis, Japan]
• GP1450 (cat), available in [wiki|Jörg Stülke]'s lab
• BKE07570 ([gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA,  downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
• BKK07570 ([gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA,  downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG

References

• 12949159,21815947,28436820,29651979,30602489