YfkN

• Description: extracellular 2',3' cyclic nucleotide phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase
  
  

Gene name	yfkN
Synonyms
Essential	yes PubMed
Product	2',3' cyclic nucleotide phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase
Function	probably phosphate acquisition
Gene expression levels in SubtiExpress: yfkN
Interactions involving this protein in SubtInteract: YfkN
MW, pI	159 kDa, 5.021
Gene length, protein length	4386 bp, 1462 aa
Immediate neighbours	hypO, yfkM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism, essential genes, membrane proteins

This gene is a member of the following regulons

CcpA regulon, PhoP regulon

The gene

Basic information

• Locus tag: BSU07840

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YhcR

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YhcS-YfkN PubMed (anchoring to the cell wall)

• Localization: extracellular (signal peptide) PubMed, the transmembrane domain serves as retention signal, attached to the cell wall PubMed

Database entries

• Structure: 3GVE

• UniProt: O34313

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfkN PubMed

• Expression browser: yfkN PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (5.2-fold) (CcpA) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional referencecs: PubMed

Hamidreza Fasehee, Helga Westers, Albert Bolhuis, Haike Antelmann, Michael Hecker, Wim J Quax, Agha F Mirlohi, Jan Maareten van Dijl, Gholamreza Ahmadian
Functional analysis of the sortase YhcS in Bacillus subtilis.
Proteomics: 2011, 11(19);3905-13
[PubMed:21800427] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

Nicholas E E Allenby, Nicola O'Connor, Zoltán Prágai, Alan C Ward, Anil Wipat, Colin R Harwood
Genome-wide transcriptional analysis of the phosphate starvation stimulon of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8063-80
[PubMed:16291680] [WorldCat.org] [DOI] (P p)

Régis Chambert, Yannick Pereira, Marie-Françoise Petit-Glatron
Purification and characterization of YfkN, a trifunctional nucleotide phosphoesterase secreted by Bacillus subtilis.
J Biochem: 2003, 134(5);655-60
[PubMed:14688230] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)