YfkM

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  • Description: glyoxalase III-like enzyme, general stress protein, survival of salt, paraquat and ethanol stresses

Gene name yfkM
Synonyms
Essential no
Product glyoxalase III-like enzyme
Function detoxification of methylglyoxal
Gene expression levels in SubtiExpress: yfkM
Metabolic function and regulation of this protein in SubtiPathways:
YfkM
MW, pI 18 kDa, 4.722
Gene length, protein length 516 bp, 172 aa
Immediate neighbours yfkN, yfkL
Sequences Protein DNA DNA_with_flanks
Genetic context
YfkM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YfkM expression.png















Categories containing this gene/protein

general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Fur regulon, SigB regulon

The gene

Basic information

  • Locus tag: BSU07850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • methylglyoxal --> D-lactate PubMed
  • Protein family: PfpI endopeptidase domain (according to Swiss-Prot)
  • Paralogous protein(s): YraA

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Pete Chandrangsu, Renata Dusi, Chris J Hamilton, John D Helmann
Methylglyoxal resistance in Bacillus subtilis: contributions of bacillithiol-dependent and independent pathways.
Mol Microbiol: 2014, 91(4);706-15
[PubMed:24330391] [WorldCat.org] [DOI] (I p)

Alexander Reder, Dirk Höper, Ulf Gerth, Michael Hecker
Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.
J Bacteriol: 2012, 194(14);3601-10
[PubMed:22582280] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol Microbiol: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)

Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166] [WorldCat.org] [DOI] (P p)