yesZ

168

beta-galactosidase, has transgalactosylation activity

locus: BSU_07080

Molecular weight: 73.92 kDa

pI: 5.5

Protein length: 663 aa

Gene length: 1992 bp

function: unknown

product: beta-galactosidase

essential: no

ec: 3.2.1.23

synonyms: yesZ

List of homologs in different organisms, belongs to COG1874 (Galperin et al., 2021)

Gene

Coordinates: 774,799  776,790

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The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to UniProt)

Protein family

• glycosyl hydrolase 42 family (with [protein|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA], according to UniProt)

Structure

• [PDB|1KWG] (from Thermus thermophilus, 31% identity) [pubmed|12215416]
• [AF|O31529]

Biological materials

Mutant

• MGNA-B455 (yesZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1454 NBRP B. subtilis, Japan]
• BKE07080 ([gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATGATACAGTTTTCTCATCA,  downstream forward: _UP4_TGATTCTTTGTATCGAATCA
• BKK07080 ([gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATGATACAGTTTTCTCATCA,  downstream forward: _UP4_TGATTCTTTGTATCGAATCA

References

• 19651770,17449691,17056685,12215416,30036621