Difference between revisions of "YesX"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
(Expression and regulation)
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' [[yesS]]-[[yesT]]-[[yesU]]-[[yesV]]-[[yesW]]-[[yesX]]-[[yesY]]-[[yesZ]]
+
* '''Operon:''' [[yesS]]-[[yesT]]-[[yesU]]-[[yesV]]-[[yesW]]-[[yesX]]-[[yesY]]-[[yesZ]] {{PubMed|19651770}}
{{PubMed|19651770}}
 
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  

Revision as of 22:05, 12 December 2009

  • Description: rhamnogalacturonanlyase, degrades oligo- to disaccharides

Gene name yesX
Synonyms
Essential no
Product rhamnogalacturonan lyase, degrades oligo- to disaccharides
Function utilization of rhamnogalacturonan
MW, pI 67 kDa, 5.223
Gene length, protein length 1836 bp, 612 aa
Immediate neighbours yesW, yesY
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YesX context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU07060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: polysaccharide lyase 11 family (according to Swiss-Prot)
  • Paralogous protein(s): YesW

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot)

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: YesS: transcriptional activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sandrine Poncet, Maryline Soret, Peggy Mervelet, Josef Deutscher, Philippe Noirot
Transcriptional activator YesS is stimulated by histidine-phosphorylated HPr of the Bacillus subtilis phosphotransferase system.
J Biol Chem: 2009, 284(41);28188-28197
[PubMed:19651770] [WorldCat.org] [DOI] (I p)

Akihito Ochiai, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
J Biol Chem: 2009, 284(15);10181-9
[PubMed:19193638] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691] [WorldCat.org] [DOI] (P p)