dnmA

dnmA
168

DNA methyltransferase, forms N6-methyladenosine at nonpalindromic 5′-GACGAG-3′ sites

locus
BSU_06760
Molecular weight
100.96 kDa
pI
5.67
Protein length
Gene length
function
genome-wide N6-methyladenosine modifications at non-palindromic recognition sites to control promoter activities
product
DNA methyltransferase
essential
no
synonyms
dnmA, yeeA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1002 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
740,288 → 742,927
additional information
the [gene|search|dmnA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC] gene cluster is a remnant of a phage defense system that no longer protects against phage predation [pubmed|36645292]
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The protein
Catalyzed reaction/ biological activity
formation of m6A DNA modifications at non-palindromic sites [pubmed|32324221]
Structure
[PDB|5HR4] (from Methylophilus methylotrophus, 38% identity) [pubmed|27082731]
[AF|O31504]
[wiki|Localization]
replisome region and the nucleoid [pubmed|36645292]
Expression and Regulation
Operons
genes
[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]-[gene|5B5014A68D777DB79179904D5FD3D14DEE0BF1F4|yefC]-[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]
description
[Pubmed|22383849]
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[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]→[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]

2024-03-10 08:58:30

Jstuelk

75

ff916330f6b5023fdc317a5fb342282b6227f373

336CDC790AAC5927B58A940F2B9646F19FD7FCCD

Biological materials
Mutant
MGNA-A959 (yeeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/959 NBRP B. subtilis, Japan]
BKE06760 (Δ[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA,  downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG
BKK06760 (Δ[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA,  downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG
References
Research papers
27082731,32324221,36645292

D3F00747006D974A8635D4391E0C261A3276E6AB

Page visits: 1812

Time of last update: 2024-03-28 09:13:42

Author of last update: Jstuelk