ydjA

168

component of the BsuM DNA restriction system

locus: BSU_06110

Molecular weight: 54.17 kDa

pI: 8.33

Protein length: 465 aa

Gene length: 1398 bp

function: BsuM DNA restriction

product: unknown

essential: no

ec: null

synonyms: ydjA

List of homologs in different organisms

Gene

Coordinates: 661,630 → 663,027

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The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)

Structure

• [AF|O34303]

Biological materials

Mutant

• MGNA-C209 (ydjA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2207 NBRP B. subtilis, Japan]
• BKE06110 (Δ[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|ydjA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAACTTACTTGACTTATCCA,  downstream forward: _UP4_TAGTTCTAGAAGGTTAAAAG
• BKK06110 (Δ[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|ydjA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAACTTACTTGACTTATCCA,  downstream forward: _UP4_TAGTTCTAGAAGGTTAAAAG

References

• 11751814,22092861