rimI

168

protein N-acetyltransferase

locus: BSU_05930

Molecular weight: 17.23 kDa

pI: 5.67

Protein length: 151 aa

Gene length: 456 bp

function: acetylation of [protein|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|S18] and [protein|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|EF-Tu]

product: protein N-acetyltransferase

essential: no

ec: 2.3.1.128

synonyms: ydiD

List of homologs in different organisms, belongs to COG0456 (Galperin et al., 2021)

Gene

Coordinates: 642,810 → 643,265

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The protein

Catalyzed reaction/ biological activity

• acetylation of [protein|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|S18] and [protein|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|EF-Tu] [pubmed|35398352]

Protein family

• [wiki|Acetyltransferase family] (according to UniProt)

[wiki|Domains]

• [wiki|N-acetyltransferase domain] (aa 5-150) (according to UniProt)

Structure

• [PDB|2CNM] [pubmed|18596200]
• [AF|O05517]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-C198 (ydiD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2196 NBRP B. subtilis, Japan]
• BKE05930 (Δ[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGCTTTTGTTTTCATCCTAT,  downstream forward: _UP4_GCGTTAATTATGTGGGTGAC
• BKK05930 (Δ[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGCTTTTGTTTTCATCCTAT,  downstream forward: _UP4_GCGTTAATTATGTGGGTGAC

References

• 36815589,22383849,18596200,35398352