Difference between revisions of "YddT"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
Line 121: Line 121:
 
* '''Mutant:'''
 
* '''Mutant:'''
 
**BP116 (spc), available in [[Fabian Commichau]]'s lab
 
**BP116 (spc), available in [[Fabian Commichau]]'s lab
 +
 
* '''Expression vector:'''
 
* '''Expression vector:'''
 +
** IPTG inducible expression of Strep-''yddT'' in ''E. coli'': pGP2581 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 16:36, 13 August 2013

  • Description: unknown essential gene

Gene name yddT
Synonyms
Essential yes PubMed
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yddT
MW, pI 25 kDa, 9.546
Gene length, protein length 684 bp, 228 aa
Immediate neighbours ydzM, ydzN
Sequences Protein DNA DNA_with_flanks
Genetic context
YddT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
500px















Categories containing this gene/protein

essential genes, proteins of unknown function

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU05100

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): almost identical to the protein YomL encoded by the prophage SPß (the two proteins differ in only 4 out of 228 amino acids)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • IPTG inducible expression of Strep-yddT in E. coli: pGP2581 (in pGP172), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Fabian M Commichau, Nico Pietack, Jörg Stülke
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
Mol Biosyst: 2013, 9(6);1068-75
[PubMed:23420519] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)