YdaP

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  • Description: general stress protein, required for survival of ethanol stress, putative pyruvate oxidase

Gene name ydaP
Synonyms
Essential no
Product putative pyruvate oxidase
Function unknown
Gene expression levels in SubtiExpress: ydaP
MW, pI 62 kDa, 5.217
Gene length, protein length 1722 bp, 574 aa
Immediate neighbours mutT, ydzK
Sequences Protein DNA DNA_with_flanks
Genetic context
YdaP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YdaP expression.png















Categories containing this gene/protein

general stress proteins (controlled by SigB), poorly characterized/ putative enzymes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU04340

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 4KGD (the enzyme from Lactobacillus plantarum, 37% identity, 70% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP457 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Danilo Meyer, Piotr Neumann, Ralf Ficner, Kai Tittmann
Observation of a stable carbene at the active site of a thiamin enzyme.
Nat Chem Biol: 2013, 9(8);488-90
[PubMed:23748673] [WorldCat.org] [DOI] (I p)

Danilo Meyer, Piotr Neumann, Eline Koers, Hanno Sjuts, Stefan Lüdtke, George M Sheldrick, Ralf Ficner, Kai Tittmann
Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin.
Proc Natl Acad Sci U S A: 2012, 109(27);10867-72
[PubMed:22730460] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166] [WorldCat.org] [DOI] (P p)