amj

168

lipid II flippase, alternative to [protein|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]

locus: BSU_04230

Molecular weight: 29.30 kDa

pI: 10.6

Protein length: 269 aa

Gene length: 810 bp

function: export of lipid II

product: lipid II flippase

essential: no

synonyms: amj, ydaH

List of homologs in different organisms

Gene

Coordinates: 474,731  475,540

Phenotypes of a mutant

• an ''[gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj] [gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]'' double mutant is not viable [Pubmed|25918422]

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The protein

Protein family

• Amj family (single member, according to UniProt)

Structure

• [AF|P96581]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj]

  description: [Pubmed|17434969]

  regulation

  • induced in the stationary phase due to the internal production of toxic peptides ([wiki|SdpC], [wiki|SkfA]) [Pubmed|26364265]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]

Biological materials

Mutant

• MGNA-C089 (ydaH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2087 NBRP B. subtilis, Japan]
• BKE04230 ([gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTTATTCTTCCTCCAAT,  downstream forward: _UP4_TAATAAGAAAGGCTGGATCA
• BKK04230 ([gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTTATTCTTCCTCCAAT,  downstream forward: _UP4_TAATAAGAAAGGCTGGATCA

References

Reviews

• 26679002,28975672,37238589

Original Publications

• 17434969,25918422,17434969,26364265