ydaF

168

similar to acetyltransferase

locus: BSU_04210

Molecular weight: 20.88 kDa

pI: 5.56

Protein length: 183 aa

Gene length: 552 bp

function: unknown

product: unknown

essential: no

synonyms: ydaF

List of homologs in different organisms, belongs to COG1670 (Galperin et al., 2021)

Gene

Coordinates: 473,174 → 473,725

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The protein

Protein family

• [wiki|Acetyltransferase family] (according to UniProt)

[wiki|Domains]

• [wiki|N-acetyltransferase domain] (aa 10-176) (according to UniProt)

Structure

• [PDB|1NSL]\t[Pubmed|15468321]
• [AF|P96579]

Paralogous protein(s)

• [protein|6072A6EF0766F3D1B1616A20CDC36CCDC6CBDA71|yjcK]

Expression and Regulation

Operons

• genes: [gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]-[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]

  description: [Pubmed|22383849]

• genes: [gene|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD]-[gene|DDE975DA2E13759B0DB8B6D219760C2E2C6032FB|ydaE]-[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]-[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]

  description: [Pubmed|10220166]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|15805528]

  expression

  • expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528,10220166], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-C088 (ydaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2086 NBRP B. subtilis, Japan]
• BKE04210 (Δ[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGCCTCGCCCTCTTTCC,  downstream forward: _UP4_TAAAGAGCACACTTTCTTGT
• BKK04210 (Δ[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGCCTCGCCCTCTTTCC,  downstream forward: _UP4_TAAAGAGCACACTTTCTTGT

References

• 10220166,18840696,15468321,36840557