ycnK

168

copper-responsive transcription repressor of the [gene|98D807FBB847BF731B5C236557C8F70347279CE0|ycnK]-[gene|D0F553E661A35C69380ACB01D9A96FC6C5088470|ycnJ]-[gene|1B7F59F954A1F20F75F5D4953F84CE58171741DE|ycnI] operon, [wiki|DeoR family]

locus: BSU_03960

Molecular weight: 21.29 kDa

pI: 7.37

Protein length: 190 aa

Gene length: 573 bp

function: regulation of copper uptake

product: transcription repressor, [wiki|DeoR family]

essential: no

synonyms: ycnK

List of homologs in different organisms, belongs to COG1349 (Galperin et al., 2021)

Gene

Coordinates: 448,461  449,033

Phenotypes of a mutant

• elevated expression of ''[gene|D0F553E661A35C69380ACB01D9A96FC6C5088470|ycnJ]'' under conditions of copper excess [Pubmed|19168619]

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The protein

Catalyzed reaction/ biological activity

• repression of ''[gene|98D807FBB847BF731B5C236557C8F70347279CE0|ycnK]-[gene|D0F553E661A35C69380ACB01D9A96FC6C5088470|ycnJ]-[gene|1B7F59F954A1F20F75F5D4953F84CE58171741DE|ycnI]'' expression in the presence of excess copper [Pubmed|22904286,19168619]

Protein family

• DeoR family of transcription factors

[wiki|Domains]

• [wiki|HTH deoR-type domain] (aa 3-58) (according to UniProt)

[wiki|Cofactors]

• copper acts as corepressor [Pubmed|19168619]

Structure

• [AF|P94433]

Biological materials

Mutant

• MGNA-C017 (ycnK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2015 NBRP B. subtilis, Japan]
• BKE03960 ([gene|98D807FBB847BF731B5C236557C8F70347279CE0|ycnK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATACACCCTCTTCAT,  downstream forward: _UP4_TAACACAACACATACAGCGG
• BKK03960 ([gene|98D807FBB847BF731B5C236557C8F70347279CE0|ycnK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATACACCCTCTTCAT,  downstream forward: _UP4_TAACACAACACATACAGCGG

labs

• [wiki|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

Reviews

• 22918892,25209494

Original Publications

• 19168619,22904286,22383849,15101989