Difference between revisions of "YcnJ"

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Revision as of 12:33, 16 May 2013

  • Description: copper transporter

Gene name ycnJ
Synonyms
Essential no
Product copper transporter
Function uptake of copper
Gene expression levels in SubtiExpress: ycnJ
MW, pI 59 kDa, 10.098
Gene length, protein length 1623 bp, 541 aa
Immediate neighbours ycnI, ycnK
Sequences Protein DNA DNA_with_flanks
Genetic context
YcnJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YcnJ expression.png















Categories containing this gene/protein

transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins

This gene is a member of the following regulons

YcnK regulon

The gene

Basic information

  • Locus tag: BSU03950

Phenotypes of a mutant

growth-defective under copper-limiting conditions PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: uptake of copper PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by copper limitation (YcnK) PubMed
    • induction of the ycnK-ycnJ-ycnI in a csoR mutant results from intracellular copper depletion due to the constitutive expression of the copZ-copA operon (copper export) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Your additional remarks

References

Kazutake Hirooka, Takayosh Edahiro, Kosuke Kimura, Yasutaro Fujita
Direct and indirect regulation of the ycnKJI operon involved in copper uptake through two transcriptional repressors, YcnK and CsoR, in Bacillus subtilis.
J Bacteriol: 2012, 194(20);5675-87
[PubMed:22904286] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Shashi Chillappagari, Marcus Miethke, Hein Trip, Oscar P Kuipers, Mohamed A Marahiel
Copper acquisition is mediated by YcnJ and regulated by YcnK and CsoR in Bacillus subtilis.
J Bacteriol: 2009, 191(7);2362-70
[PubMed:19168619] [WorldCat.org] [DOI] (I p)