thrD

168

aspartokinase III

locus: BSU_03790

Molecular weight: 49.66 kDa

pI: 4.78

Protein length: 454 aa

Gene length: 1365 bp

function: biosynthesis of threonine

product: aspartokinase III

essential: no

ec: 2.7.2.4

synonyms: thrD, yclM

List of homologs in different organisms, belongs to COG0527 (Galperin et al., 2021)

Gene

Coordinates: 430,623  431,987

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The protein

Catalyzed reaction/ biological activity

• ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)

Protein family

• aspartokinase family (with [protein|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG] and [protein|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC], according to UniProt)

[wiki|Domains]

• 2 [wiki|ACT domain]s (aa 312-388, aa 389-454) (according to UniProt)

Structure

• [PDB|3TVI] (from Clostridium acetobutylicum, 37% identity) [pubmed|25170437]
• [AF|P94417]

Effectors of protein activity

• inhibited by the simultaneous presence of threonine and lysine [Pubmed|11471740]

Biological materials

Mutant

• MGNA-C009 (yclM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2007 NBRP B. subtilis, Japan]
• BKE03790 (Δ[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG,  downstream forward: _UP4_TAATCGTACATAAATAGCGG
• BKK03790 (Δ[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG,  downstream forward: _UP4_TAATCGTACATAAATAGCGG

lacZ fusion

• pBP318 (in [wiki|pAC5]), available in [wiki|Fabian Commichau]'s lab [Pubmed|27260660]

References

Reviews

• 19946135,22079167

Original Publications

• 11471740,23569278,2152900,2153658,22383849,27260660,25170437