thrD

thrD
168

aspartokinase III

locus
BSU_03790
Molecular weight
49.66 kDa
pI
4.78
Protein length
Gene length
function
biosynthesis of threonine
product
aspartokinase III
essential
no
ec
2.7.2.4
synonyms
thrD, yclM

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0527 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
430,623  431,987
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The protein
Catalyzed reaction/ biological activity
ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)
Protein family
aspartokinase family (with [protein|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG] and [protein|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC], according to UniProt)
[wiki|Domains]
2 [wiki|ACT domain]s (aa 312-388, aa 389-454) (according to UniProt)
Structure
[PDB|3TVI] (from Clostridium acetobutylicum, 37% identity) [pubmed|25170437]
[AF|P94417]
Effectors of protein activity
inhibited by the simultaneous presence of threonine and lysine [Pubmed|11471740]
Expression and Regulation
Operons
genes
[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]
description
[Pubmed|22383849]
regulation
expressed in the presence of lysine or cysteine ([wiki|ThrR]) [Pubmed|27260660]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|23569278], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]: repression, [Pubmed|27260660], in [regulon|protein:A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR regulon]
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[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]

2024-03-13 20:43:18

ghost

50

200fc90f351788babff94caab54425b25ea13cbf

1170A29818E75556ED4141D0705F3CBBBF4C7AF3

Biological materials
Mutant
MGNA-C009 (yclM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2007 NBRP B. subtilis, Japan]
BKE03790 (Δ[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG,  downstream forward: _UP4_TAATCGTACATAAATAGCGG
BKK03790 (Δ[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG,  downstream forward: _UP4_TAATCGTACATAAATAGCGG
lacZ fusion
pBP318 (in [wiki|pAC5]), available in [wiki|Fabian Commichau]'s lab [Pubmed|27260660]
References
Reviews
19946135,22079167
Original Publications
11471740,23569278,2152900,2153658,22383849,27260660,25170437

548E92526BC00999031D6EDE43FE061CE0448AFC

Page visits: 2452

Time of last update: 2024-03-28 12:51:55

Author of last update: Jstuelk