yclJ

168

two-component response regulator ([wiki|OmpR family]), active under anaerobic conditions

locus: BSU_03750

Molecular weight: 26.36 kDa

pI: 4.58

Protein length: 227 aa

Gene length: 684 bp

function: unknown

product: two-component response regulator ([wiki|OmpR family])

essential: no

synonyms: yclJ

List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 426,577  427,260

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The protein

Protein family

• [wiki|OmpR family] of two-component response regulators

[wiki|Domains]

• [wiki|Response regulatory domain] (aa 2-115) (according to UniProt)

Structure

• [PDB|2OQR] (RegX3 from Mycobacterium tuberculosis, 42% identity) [pubmed|17942407]
• [AF|P94413]

Modification

• phosphorylated by [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Paralogous protein(s)

• [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR], [protein|6A37531896205A9B894C81AB0563C216C0B52CD7|ykoG], [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-C007 (yclJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2005 NBRP B. subtilis, Japan]
• BKE03750 ([gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT,  downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA
• BKK03750 ([gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT,  downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA

References

• 10094672,20512483,15375128,23504016,17942407