yciB

168

putative L,D-transpeptidase

locus: BSU_03350

Molecular weight: 21.94 kDa

pI: 6.18

Protein length: 194 aa

Gene length: 585 bp

function: unknown

product: putative L,D-transpeptidase

essential: no

synonyms: yciB

List of homologs in different organisms, belongs to COG1376 (Galperin et al., 2021)

Gene

Coordinates: 365,170  365,754

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The protein

Protein family

• YkuD family (with [protein|6F256986E591DB82974A7F54EB1CE0C024A6F63B|ldt] and [protein|293794B65B9EEB7F049138F73893303BABFB9A2A|yqjB], according to UniProt)

Structure

• [PDB|4LZH] (from Klebsiella pneumoniae, 27% identity)
• [AF|C0SP99]

[wiki|Localization]

• lipoprotein, cell membrane at the cell poles [Pubmed|20013255]

Biological materials

Mutant

• MGNA-B998 (yciB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1997 NBRP B. subtilis, Japan]
• BKE03350 ([gene|456AF184261976FAA73359E81443F7035F457D07|yciB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGCAATAATGAATAATGAGA,  downstream forward: _UP4_TGAATTGAAAACCGTTGACA
• BKK03350 ([gene|456AF184261976FAA73359E81443F7035F457D07|yciB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGCAATAATGAATAATGAGA,  downstream forward: _UP4_TGAATTGAAAACCGTTGACA

References

• 12426338,20013255,9811636